TRAFD1 Polyklonaler Antikörper

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• KD/KO Validated

TRAFD1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27741-1-AP

Synonyme

TRAF-type zinc finger domain-containing protein 1, Protein FLN29, FLN29

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  WB analysis of HEK-293 using 27741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27741-1-AP

  WB result of TRAFD1 antibody (27741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAFD1 transfected A549 cells.

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  WB analysis of HeLa using 27741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 27741-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 27741-1-AP

  IP result of anti-TRAFD1 (IP:27741-1-AP, 4ug; Detection:27741-1-AP 1:800) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27741-1-AP bindet in WB, IHC, IP, ELISA TRAFD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAFD1 fusion protein Ag26945
• Vollständiger Name: TRAF-type zinc finger domain containing 1
• Berechnetes Molekulargewicht: 582 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-80 kDa
• GenBank-Zugangsnummer: BC003553
• Gene symbol: TRAFD1
• Gene ID (NCBI): 10906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAFD1, also known as TRAF-type zinc finger domain containing 1, is a protein that plays a significant role in immune system signaling pathways. It was first identified as an interferon- (IFN) and lipopolysaccharide- (LPS) inducible factor. TRAFD1 contains a TRAF-type zinc finger domain at its N-terminus and a TRAF6-binding motif in its middle region, which allows it to interact with other TRAF proteins and modulate immune responses. In the context of immune signaling, TRAFD1 has been shown to suppress the inflammatory responses to innate immunity by inhibiting Toll-like receptor 4 (TLR4) dependent NF-κB and MAPK activation in monocytes/macrophages. This suggests that TRAFD1 can act as a negative regulator of immune signaling, dampening the inflammatory response. Moreover, TRAFD1 has been implicated in human diseases. It is overexpressed in many B cell-related cancers, and single nucleotide polymorphisms (SNPs) in TRAFD1 have been linked to non-Hodgkin's lymphoma.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator; Authors - Na Jiang