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  • KD/KO Validated

TRAIL/CD253 Polyklonaler Antikörper

TRAIL/CD253 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 27064-1-AP

Synonyme

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhuman milk
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27064-1-AP bindet in WB, IHC, IF/ICC, ELISA TRAIL/CD253 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRAIL/CD253 fusion protein Ag25746
Vollständiger Name tumor necrosis factor (ligand) superfamily, member 10
Berechnetes Molekulargewicht 281 aa, 33 kDa
Beobachtetes Molekulargewicht 33 kDa, 20 kDa
GenBank-ZugangsnummerBC032722
Gene symbol TRAIL
Gene ID (NCBI) 8743
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TRAIL/CD253 antibody 27064-1-APProtokoll herunterladen
IHC protocol for TRAIL/CD253 antibody 27064-1-APProtokoll herunterladenl
IF protocol for TRAIL/CD253 antibody 27064-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Stem Cells Transl Med

Site-Specific Integration of TRAIL in iPSC-Derived Mesenchymal Stem Cells for Targeted Cancer Therapy.

Authors - Zujia Wang
humanWB

Int J Pharm

Fusion of an EGFR-antagonistic affibody enhances the anti-tumor effect of TRAIL to EGFR positive tumors.

Authors - Dianlong Jia
humanWB

Arthritis Res Ther

IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes.

Authors - Xiaomei Yang
humanWB

Cell Biosci

Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis.

Authors - Chuan Yang
  • KD Validated
humanWB

J Nat Prod

Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission

Authors - Xiao-Jie Chen
humanWB

Phytomedicine

Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis

Authors - Li Zhang