TRAIL/CD253 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRAIL/CD253 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27064-1-PBS

Synonyme

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL

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  WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

  Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

  Human milk were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27064-1-AP (TRAIL/CD253 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27064-1-AP (TRAIL/CD253 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 27064-1-AP (same clone as 27064-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27064-1-PBS bindet in WB, IHC, IF/ICC, ELISA TRAIL/CD253 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAIL/CD253 fusion protein Ag25746
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 10
• Berechnetes Molekulargewicht: 281 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 20 kDa
• GenBank-Zugangsnummer: BC032722
• Gene symbol: TRAIL
• Gene ID (NCBI): 8743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.