TRAIL/CD253 Monoklonaler Antikörper

TRAIL/CD253 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9B4

Kat-Nr. : 66756-1-PBS

Synonyme

TNFSF10, TRAIL, 1B9B4, Apo-2 ligand, CD253

---

• 

  WB analysis of human milk using 66756-1-Ig (same clone as 66756-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66756-1-Ig (TRAIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50341-1

  Cytometric bead array standard curve of MP50341-1, TRAIL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66756-1-PBS. Detection antibody: 66756-2-PBS. Standard:Ag25746. Range: 3.125-200 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

66756-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA TRAIL/CD253 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRAIL/CD253 fusion protein Ag25746
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 10
• Berechnetes Molekulargewicht: 281 aa, 33 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC032722
• Gene symbol: TRAIL
• Gene ID (NCBI): 8743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFSF10/TRAIL (tumor necrosis factor superfamily member 10) is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TNFSF10 induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.