TRAIL/CD253 Rekombinanter Antikörper

TRAIL/CD253 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241419D2

Kat-Nr. : 98139-1-PBS

Synonyme

TNFSF10, TRAIL, 241419D2, Apo-2 ligand, CD253

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  FC experiment of human PBMCs using 98139-1-RR (same clone as 98139-1-PBS)

  1x10^6 untreated or IFN-α treated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98139-1-RR (same clone as 98139-1-PBS)

  1x10^6 IFN-α treated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98139-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98139-1-PBS bindet in FC TRAIL/CD253 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 10
• Berechnetes Molekulargewicht: 281 aa, 33 kDa
• GenBank-Zugangsnummer: BC032722
• Gene symbol: TRAIL
• Gene ID (NCBI): 8743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.