TRAIL/CD253 Rekombinanter Antikörper

TRAIL/CD253 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241419D2

Kat-Nr. : 98139-1-RR

Synonyme

TNFSF10, TRAIL, 241419D2, Apo-2 ligand, CD253

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  FC experiment of human PBMCs using 98139-1-RR

  1x10^6 untreated or IFN-α treated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98139-1-RR

  1x10^6 IFN-α treated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit RecAb (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98139-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: IFN-alpha treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98139-1-RR bindet in FC TRAIL/CD253 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 10
• Berechnetes Molekulargewicht: 281 aa, 33 kDa
• GenBank-Zugangsnummer: BC032722
• Gene symbol: TRAIL
• Gene ID (NCBI): 8743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.