TRAIL/CD253 Rekombinanter Antikörper

TRAIL/CD253 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241419D2

Kat-Nr. : CL647-98139

Synonyme

TNFSF10, TRAIL, 241419D2, Apo-2 ligand, CD253

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  FC experiment of human PBMCs using CL647-98139

  1x10^6 untreated or IFN-a treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human TRAIL/CD253 Rabbit RecAb (CL647-98139, Clone:241419D2). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98139

  1x10^6 IFN-a treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human TRAIL/CD253 Rabbit RecAb (CL647-98139, Clone: 241419D2 (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human TRAIL/CD253 Rabbit RecAb (CL647-98139, Clone: 241419D2) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: IFN-a treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98139 bindet in FC TRAIL/CD253 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 10
• Berechnetes Molekulargewicht: 281 aa, 33 kDa
• GenBank-Zugangsnummer: BC032722
• Gene symbol: TRAIL
• Gene ID (NCBI): 8743
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.