DcR2 Rekombinanter Antikörper

DcR2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242916C11

Kat-Nr. : 85425-2-PBS

Synonyme

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

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  WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

  Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01996-1

  Sandwich ELISA standard curve of MP01996-1, Human DcR2 Recombinant Matched Antibody Pair - PBS only. 85425-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3487. 85425-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP01996-1

  The mean DcR2 concentration was determined to be 8.72 ng/mL in HCT116 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01996-1

  Cytometric bead array standard curve of MP01996-1, DcR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85425-1-PBS. Detection antibody: 85425-2-PBS. Standard: Eg3487. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85425-2-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test DcR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DcR2 fusion protein Eg3487
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_003840.5
• Gene symbol: DcR2
• Gene ID (NCBI): 8793
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).