TRAIP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRAIP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 10332-1-AP

Synonyme

TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein

---

• 

  WB analysis of mouse brain using 10332-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10332-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10332-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10332-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10332-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10332-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 10332-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10332-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10332-1-AP

  Immunofluorescent analysis of HepG2 cells using 10332-1-AP (TRAIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Produktinformation

10332-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TRAIP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAIP fusion protein Ag0491
• Vollständiger Name: TRAF interacting protein
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC000310
• Gene symbol: TRAIP
• Gene ID (NCBI): 10293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E3 ubiquitin-protein ligase TRAIP (TRAIP) is also named RING finger protein 206 (RNF206) and TRAF-interacting protein (TRIP). TRAIP, an E3 ligase containing a RING domain, has emerged as a significant contributor to maintaining genome integrity and TRAIP inhibits proliferation and migration of BLCA cells (PMID: 38123820). TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-protein crosslinks, whereas in mitosis it triggers disassembly of all replisomes that remain on chromatin (PMID: 33317933, PMID: 30842657).

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
mouse	WB	Sci Rep; Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway.; Authors - Chengcheng Li
human	WB,IHC	Biomed Pharmacother; TRAIP promotes malignant behaviors and correlates with poor prognosis in liver cancer.; Authors - Zhenya Guo ; KD Validated
mouse,human	WB,IHC	Int Urol Nephrol; TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation; Authors - Yuan Gu ; KD Validated
human	WB,IP,IHC	Cell Death Dis; TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation.; Authors - Mi Li ; KD Validated
human	WB,IHC	Cancer Gene Ther; Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT; Authors - Yan Zheng ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}