TRAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRAP1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10325-1-AP

Synonyme

HSP 75, HSP75, HSP90L, TNFR associated protein 1, TRAP 1, TRAP1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10325-1-AP

  WB result of TRAP1 antibody (10325-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells.

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  WB analysis using 10325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10325-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10325-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 10325-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAP1 antibody (10325-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10325-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TRAP1 antibody (10325-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Mausherzgewebe, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

10325-1-AP bindet in WB, IHC, IF, FC, ELISA TRAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAP1 fusion protein Ag0400
• Vollständiger Name: TNF receptor-associated protein 1
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa, 60 kDa
• GenBank-Zugangsnummer: BC000406
• Gene symbol: TRAP1
• Gene ID (NCBI): 10131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Publikationen

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
mouse	WB,IF	Glia; The LncRNA AK018453 regulates TRAP1/Smad signaling in IL-17-activated astrocytes: A potential role in EAE pathogenesis.; Authors - Qingxiu Zhang ; KD Validated
mouse	WB	J Gerontol A Biol Sci Med Sci; Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle.; Authors - Yuki Tamura
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IF	Cell Signal; Downregulated fat mass and obesity-associated protein inhibits bone resorption and osteoclastogenesis by nuclear factor-kappa B inactivation; Authors - Jinpeng Zhuang
mouse	WB	IUBMB Life; Loss of high-temperature requirement protein A2 protease activity induces mitonuclear imbalance via differential regulation of mitochondrial biogenesis in sarcopenia.; Authors - Haohan Zhou