TRAP1 Monoklonaler Antikörper

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• KD/KO Validated

TRAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H5D4

Kat-Nr. : 67693-1-PBS

Synonyme

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

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  WB analysis using 67693-1-Ig (same clone as 67693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

  WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 67693-1-Ig (same clone as 67693-1-PBS)

  IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67693-1-Ig (same clone as 67693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67693-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TRAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRAP1 fusion protein Ag0400
• Vollständiger Name: TNF receptor-associated protein 1
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC000406
• Gene symbol: TRAP1
• Gene ID (NCBI): 10131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).