TRAPB/SSR2 Polyklonaler Antikörper

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• KD/KO Validated

TRAPB/SSR2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10278-1-PBS

Synonyme

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  WB analysis using 10278-1-AP (same clone as 10278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 10278-1-AP (same clone as 10278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 10278-1-AP (same clone as 10278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10278-1-AP (same clone as 10278-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAPB/SSR2 antibody (10278-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10278-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRAPB/SSR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPB/SSR2 fusion protein Ag0304
• Vollständiger Name: signal sequence receptor, beta (translocon-associated protein beta)
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC000341
• Gene symbol: TRAPB
• Gene ID (NCBI): 6746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

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