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TRAPPC1 Polyklonaler Antikörper

TRAPPC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13868-1-AP

Synonyme

Trafficking protein particle complex subunit 1, MUM-2, MUM2, Multiple myeloma protein 2, BET5 homolog

Filter:
  • Western Blot (WB) analysis of various lysates using TRAPPC1 Polyclonal antibody (13868-1-AP)

    WB analysis using 13868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13868-1-AP (TRAPPC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAPPC1 Polyclonal antibody (13868-1-AP)

    IHC staining of mouse brain using 13868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13868-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAPPC1 Polyclonal antibody (13868-1-AP)

    IHC staining of mouse brain using 13868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13868-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAPPC1 Polyclonal antibody (13868-1-AP)

    IF Staining of HeLa using 13868-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC1 antibody (13868-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13868-1-AP bindet in WB, IHC, IF/ICC, ELISA TRAPPC1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRAPPC1 fusion protein Ag4616
Vollständiger Name trafficking protein particle complex 1
Berechnetes Molekulargewicht 145 aa, 17 kDa
Beobachtetes Molekulargewicht 15-17 kDa
GenBank-ZugangsnummerBC032717
Gene symbol TRAPPC1
Gene ID (NCBI) 58485
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC1, also known as BET5 or MUM2, is a part of the multisubunit TRAPP (transport protein particle) complexes which are involved in the tethering process at different trafficking steps of vesicle transport (PMID: 16828797). TRAPPC1 may play a role in vesicular transport from the endoplasmic reticulum to the Golgi (PMID: 10582700).