TRAPPC10 Polyklonaler Antikörper

TRAPPC10 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27549-1-PBS

Synonyme

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  WB analysis using 27549-1-AP (same clone as 27549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

  IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 27549-1-AP (same clone as 27549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27549-1-PBS bindet in WB, IHC, IP, Indirect ELISA TRAPPC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPPC10 fusion protein Ag26900
• Vollständiger Name: trafficking protein particle complex 10
• Berechnetes Molekulargewicht: 1259 aa, 142 kDa
• Beobachtetes Molekulargewicht: 142 kDa
• GenBank-Zugangsnummer: BC101728
• Gene symbol: TRAPPC10
• Gene ID (NCBI): 7109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.