TRAPPC12 Polyklonaler Antikörper

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• KD/KO Validated

TRAPPC12 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24555-1-AP

Synonyme

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

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  WB analysis using 24555-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24555-1-AP

  WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells.

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  IP experiment of K-562 using 24555-1-AP

  IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24555-1-AP bindet in WB, IP, ELISA TRAPPC12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPPC12 fusion protein Ag18332
• Vollständiger Name: tetratricopeptide repeat domain 15
• Berechnetes Molekulargewicht: 735 aa, 79 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC014164
• Gene symbol: TRAPPC12
• Gene ID (NCBI): 51112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).