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TRAPPC12 Polyklonaler Antikörper

TRAPPC12 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24555-1-PBS

Synonyme

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

Filter:
  • Western Blot (WB) analysis of various lysates using TRAPPC12 Polyclonal antibody (24555-1-AP)

    WB analysis using 24555-1-AP (same clone as 24555-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TRAPPC12 Polyclonal antibody (24555-1-AP)

    WB analysis of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using TRAPPC12 Polyclonal antibody (24555-1-AP)

    IP experiment of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24555-1-PBS bindet in WB, IP, Indirect ELISA TRAPPC12 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRAPPC12 fusion protein Ag18332
Vollständiger Name tetratricopeptide repeat domain 15
Berechnetes Molekulargewicht 735 aa, 79 kDa
Beobachtetes Molekulargewicht98 kDa
GenBank-ZugangsnummerBC014164
Gene symbol TRAPPC12
Gene ID (NCBI) 51112
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).