TRAPPC3 Polyklonaler Antikörper

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• KD/KO Validated

TRAPPC3 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15555-1-AP

Synonyme

BET3, BET3 homolog, TRAPPC3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 15555-1-AP

  WB result of TRAPPC3 antibody (15555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC3 transfected HEK-293 cells.

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  WB analysis of mouse liver using 15555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 15555-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15555-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15555-1-AP

  IP Result of anti-TRAPPC3 (IP:15555-1-AP, 3ug; Detection:15555-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15555-1-AP

  Immunofluorescent analysis of Hela cells, using TRAPPC3 antibody 15555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, Maus-Dünndarmgewebe, PC-3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

15555-1-AP bindet in WB, IP, IHC, IF, ELISA TRAPPC3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPPC3 fusion protein Ag7924
• Vollständiger Name: trafficking protein particle complex 3
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC007662
• Gene symbol: TRAPPC3
• Gene ID (NCBI): 27095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)

Publikationen

Species	Application	Title
human	WB	Cell; A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.; Authors - Bassik Michael C MC
human	WB	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
mouse	WB	Int J Biol Sci; Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice; Authors - Min Hu
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca
human	WB	Signal Transduct Target Ther; RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.; Authors - Yalan Lu