TRBP Polyklonaler Antikörper

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• KD/KO Validated

TRBP Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 15753-1-AP

Synonyme

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15753-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15753-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15753-1-AP

  IP Result of anti-TRBP (IP:15753-1-AP, 4ug; Detection:15753-1-AP 1:1500) with mouse liver tissue lysate 6400ug.

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  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15753-1-AP

  Immunofluorescent analysis of Hela cells, using TARBP2 antibody 15753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, Mausmilzgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

15753-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA TRBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRBP fusion protein Ag8255
• Vollständiger Name: TAR (HIV-1) RNA binding protein 2
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 43-46 kDa
• GenBank-Zugangsnummer: BC005860
• Gene symbol: TARBP2
• Gene ID (NCBI): 6895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

Publikationen

Species	Application	Title
human	WB,IF	Mol Cell; Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.; Authors - Lisa Fish ; KD Validated
mouse	WB	Mol Cell; A Rapid Induction Mechanism for Lin28a in Trophic Responses.; Authors - Alexandra M Amen
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human	WB	Nat Commun; SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.; Authors - Cheng Chen ; KD Validated
human	WB,IF	Nucleic Acids Res; S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.; Authors - Matthew J Warner ; KD Validated
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou