TREM2 Rekombinanter Antikörper

TREM2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

240384B7

Kat-Nr. : 83438-6-RR

Synonyme

240384B7, TREM 2, TREM-2, Trem2a, Trem2b

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  WB analysis of THP-1 using 83438-6-RR

  PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 83438-6-RR

  Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83438-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83438-6-RR against Human TREM2 were performed. The affinity constant is 38.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA treated THP-1 cells, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

83438-6-RR bindet in WB, ELISA TREM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TREM2 fusion protein Eg0747
• Vollständiger Name: triggering receptor expressed on myeloid cells 2
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 26-32 kDa
• GenBank-Zugangsnummer: BC032362
• Gene symbol: TREM2
• Gene ID (NCBI): 54209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; TREM2 alleviates sepsis-induced acute lung injury by attenuating ferroptosis via the SHP1/STAT3 pathway; Authors - Siyi Wu
mouse	WB	ACS Infect Dis; A Photoactivatable Free Mycolic Acid Probe to Investigate Mycobacteria-Host Interactions; Authors - Kingsley C Agu