TRIB3 Monoklonaler Antikörper

TRIB3 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2C10C7

Kat-Nr. : 66702-1-Ig

Synonyme

C20orf97, NIPK, SINK, SKIP3, TRB 3, TRB3, TRIB3, Tribbles homolog 3

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Galerie der Validierungsdaten

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  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 66702-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66702-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66702-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse hepatocytes using 66702-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, DU 145-Zellen, HepG2-Zellen, HUVEC-Zellen, L02-Zellen, LNCaP-Zellen, MCF-7-Zellen, PC-3-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66702-1-Ig bindet in WB, ELISA TRIB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIB3 fusion protein Ag25776
• Vollständiger Name: tribbles homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 358 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC027484
• Gene symbol: TRIB3
• Gene ID (NCBI): 57761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Publikationen

Species	Application	Title
mouse	WB	JCI Insight; Mitochondrial and NAD+ metabolism predict recovery from acute kidney injury in a diverse mouse population; Authors - Jean-David Morel
human	WB	Drug Des Devel Ther; PMID: 31118581; Authors - Hang Zhao