TRIM14 Polyklonaler Antikörper

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TRIM14 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15742-1-PBS

Synonyme

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  WB analysis using 15742-1-AP (same clone as 15742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 15742-1-AP (same clone as 15742-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15742-1-AP (same clone as 15742-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM14 antibody (15742-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15742-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRIM14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM14 fusion protein Ag8158
• Vollständiger Name: tripartite motif-containing 14
• Berechnetes Molekulargewicht: 442 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006333
• Gene symbol: TRIM14
• Gene ID (NCBI): 9830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.