TRIM21 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM21 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, coIP, ELISA

Konjugation

Unkonjugiert

Publikationen

53

Rezensionen (4)

Kat-Nr. : 12108-1-AP

Synonyme

52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21

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Galerie der Validierungsdaten

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  WB analysis using 12108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12108-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12108-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12108-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12108-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12108-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP (TRIM21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12108-1-AP

  IP Result of anti-TRIM21 (IP:12108-1-AP, 4ug; Detection:12108-1-AP 1:500) with A549 cells lysate 920ug.

• 

  IHC staining of human pancreas cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12108-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12108-1-AP (TRIM21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 12108-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM21 antibody (12108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, HL-60-Zellen, HT-1080.Zellen, HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 21 publications below
• WB: See 46 publications below
• IHC: See 14 publications below
• IF: See 16 publications below
• IP: See 15 publications below
• CoIP: See 7 publications below

Produktinformation

12108-1-AP bindet in WB, IP, IHC, IF, coIP, ELISA TRIM21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM21 fusion protein Ag2753
• Vollständiger Name: tripartite motif-containing 21
• Berechnetes Molekulargewicht: 475 aa, 54 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa, 49-54 kDa
• GenBank-Zugangsnummer: BC010861
• Gene symbol: TRIM21
• Gene ID (NCBI): 6737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

Publikationen

Species	Application	Title
human	IHC	J Clin Invest; The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain-of-function in cancer; Authors - Juan Liu
human	WB	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang ; KD Validated
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
mouse	IF	Transl Res; MECHANISM OF ACTION OF IC 100, A HUMANIZED IgG4 MONOCLONAL ANTIBODY TARGETING APOPTOSIS-ASSOCIATED SPECK-LIKE STAINING PROTEIN CONTAINING A CASPASE RECRUITMENT DOMAIN (ASC).; Authors - Juan Pablo de Rivero Vaccari
human	WB	Proc Natl Acad Sci U S A; Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein.; Authors - Jack Chun-Chieh Hsu
human	WB,coIP	EMBO Rep; TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity.; Authors - Zhaolong Li ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (01-14-2020)

Used routinely for Western Blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEKs

FH

Laura (Verified Customer) (01-14-2020)

Gives a clean Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (01-10-2020)

Used many times in many WB and IF applications. Reliably antibody which detects TRIM21 basal as well as fusion proteins in WB and can be used to visualise TRIM21 in cells through IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500 WB, 1:200 IF
• Cell Tissue Type: HEK, SHSY5Y

FH

Alinda (Verified Customer) (09-11-2019)

HEK were transfected with a TRIM21 overexpression vector- secondary anitbody used was rb Alexa 488.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK-293T

TRIM21 Antibody Immunofluorescence, validation (1:200 dilution) in HEK-293T (Cat no:12108-1-AP)

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