TRIM22 Rekombinanter Antikörper

TRIM22 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240411D6

Kat-Nr. : 83479-4-PBS

Synonyme

RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

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  WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

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  WB analysis using 83479-4-RR (same clone as 83479-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83479-4-RR (same clone as 83479-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM22 antibody (83479-4-RR, Clone: 240411D6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83479-4-RR (same clone as 83479-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug TRIM22 Recombinant antibody (83479-4-RR, Clone:240411D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83479-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83479-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83479-4-RR against Human TRIM22 were performed. The affinity constant is 0.181 nM.

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Geprüfte Anwendungen

Produktinformation

83479-4-PBS bindet in WB, IF/ICC, FC (Intra), ELISA TRIM22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM22 fusion protein Ag4683
• Vollständiger Name: tripartite motif-containing 22
• Berechnetes Molekulargewicht: 498 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC035582
• Gene symbol: TRIM22
• Gene ID (NCBI): 10346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid