TRIM24 Monoklonaler Antikörper

TRIM24 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D6D9

Kat-Nr. : 66324-1-Ig

Synonyme

1D6D9, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1

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  WB analysis using 66324-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66324-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66324-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66324-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM24 antibody (66324-1-Ig, Clone: 1D6D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66324-1-Ig bindet in WB, IHC, IF/ICC, ELISA TRIM24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM24 fusion protein Ag5417
• Vollständiger Name: tripartite motif-containing 24
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC028689
• Gene symbol: TRIM24
• Gene ID (NCBI): 8805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.