TRIM24 Monoklonaler Antikörper

TRIM24 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D6D9

Kat-Nr. : 66324-1-PBS

Synonyme

1D6D9, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1

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  WB analysis using 66324-1-Ig (same clone as 66324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66324-1-Ig (same clone as 66324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66324-1-Ig (same clone as 66324-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM24 antibody (66324-1-Ig, Clone: 1D6D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66324-1-PBS bindet in WB, IHC, IF/ICC, ELISA TRIM24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM24 fusion protein Ag5417
• Vollständiger Name: tripartite motif-containing 24
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC028689
• Gene symbol: TRIM24
• Gene ID (NCBI): 8805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.