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TRIM25 Polyklonaler Antikörper
TRIM25 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12573-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen |
| Erfolgreiche IP | K-562-Zellen |
| Erfolgreiche Detektion in IHC | humanes Nierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 33 publications below |
| IF | See 10 publications below |
| IP | See 5 publications below |
| CoIP | See 3 publications below |
Produktinformation
12573-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TRIM25 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Affe |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | TRIM25 fusion protein Ag3241 |
| Vollständiger Name | tripartite motif-containing 25 |
| Berechnetes Molekulargewicht | 630 aa, 71 kDa |
| Beobachtetes Molekulargewicht | 68-71 kDa |
| GenBank-Zugangsnummer | BC016924 |
| Gene symbol | TRIM25 |
| Gene ID (NCBI) | 7706 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for TRIM25 antibody 12573-1-AP | Protokoll herunterladen |
| IHC protocol for TRIM25 antibody 12573-1-AP | Protokoll herunterladenl |
| IF protocol for TRIM25 antibody 12573-1-AP | Protokoll herunterladen |
| IP protocol for TRIM25 antibody 12573-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis | ||
Adv Sci (Weinh) Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy | ||
Cell Rep p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP | ||
Elife Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses. | ||
Haematologica Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iram (Verified Customer) (01-15-2021) | TRIM25 antibody giving a very clear and sharp expression.
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