TRIM25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM25 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Rezensionen (1)

Kat-Nr. : 12573-1-AP

Synonyme

EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase

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  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12573-1-AP

  IP result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.

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  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IF: See 10 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Produktinformation

12573-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TRIM25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM25 fusion protein Ag3241
• Vollständiger Name: tripartite motif-containing 25
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 68-71 kDa
• GenBank-Zugangsnummer: BC016924
• Gene symbol: TRIM25
• Gene ID (NCBI): 7706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

Publikationen

Species	Application	Title
	WB	Signal Transduct Target Ther; Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis; Authors - Yuanliang Yan
human	WB	Adv Sci (Weinh); Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy; Authors - Zhao-Xin Zhang
human	WB	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang
human	WB	Elife; Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.; Authors - Wei Liu
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer cells and tissue