TRIM25 Monoklonaler Antikörper

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• KD/KO Validated

TRIM25 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6G1

Kat-Nr. : 67314-1-PBS

Synonyme

1H6G1, EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein

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  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67314-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRIM25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM25 fusion protein Ag29325
• Vollständiger Name: tripartite motif-containing 25
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 68-71 kDa
• GenBank-Zugangsnummer: BC016924
• Gene symbol: TRIM25
• Gene ID (NCBI): 7706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).