TRIM25 Rekombinanter Antikörper

TRIM25 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250176H6

Kat-Nr. : 85919-1-PBS

Synonyme

EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase

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  WB analysis using 85919-1-RR (same clone as 85919-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85919-1-RR (TRIM25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85919-1-RR (same clone as 85919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85919-1-RR (TRIM25 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85919-1-RR (same clone as 85919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85919-1-RR (TRIM25 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 85919-1-RR (same clone as 85919-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM25 antibody (85919-1-RR, Clone: 250176H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85919-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85919-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRIM25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM25 fusion protein Ag29325
• Vollständiger Name: tripartite motif-containing 25
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC016924
• Gene symbol: TRIM25
• Gene ID (NCBI): 7706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).