TRIM26 Rekombinanter Antikörper

TRIM26 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242154B5

Kat-Nr. : 84661-4-RR

Synonyme

242154B5, EC:2.3.2.27, RNF95, TRIM, Tripartite motif-containing protein 26

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  WB analysis using 84661-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84661-4-RR (TRIM26 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of human rectal cancer using 84661-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84661-4-RR (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 84661-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84661-4-RR (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84661-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84661-4-RR against Human TRIM26 were performed. The affinity constant is 47.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: human rectal cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84661-4-RR bindet in WB, IHC, ELISA TRIM26 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM26 fusion protein Ag25546
• Vollständiger Name: tripartite motif-containing 26
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC024039
• Gene symbol: TRIM26
• Gene ID (NCBI): 7726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)