TRIM29 Rekombinanter Antikörper

TRIM29 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251298E11

Kat-Nr. : 86496-1-RR

Synonyme

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

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  WB analysis of HT-29 using 86496-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 86496-1-RR (TRIM29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86496-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86496-1-RR (TRIM29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86496-1-RR against Human TRIM29 were performed. The affinity constant is 0.424 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, A431-Zellen, BGC-823-Zellen, HaCaT-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86496-1-RR bindet in WB, ELISA TRIM29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM29 fusion protein Ag11600
• Vollständiger Name: tripartite motif-containing 29
• Berechnetes Molekulargewicht: 588 aa, 66 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC017352
• Gene symbol: TRIM29
• Gene ID (NCBI): 23650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).