TRIM31 Polyklonaler Antikörper

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• KD/KO Validated

TRIM31 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12543-1-PBS

Synonyme

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  WB analysis of COLO 320 using 12543-1-AP (same clone as 12543-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12543-1-AP (TRIM31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12543-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HT-29 using 12543-1-AP (same clone as 12543-1-PBS)

  IP result of anti-TRIM31 (IP:12543-1-AP, 4ug; Detection:12543-1-AP 1:2000) with HT-29 cells lysate 1040 ug. This data was developed using the same antibody clone with 12543-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 12543-1-AP (same clone as 12543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12543-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 12543-1-AP (same clone as 12543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Colon cancer using 12543-1-AP (same clone as 12543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 12543-1-AP (TRIM31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12543-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12543-1-PBS bindet in WB, IHC, IP, Indirect ELISA TRIM31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM31 fusion protein Ag3264
• Vollständiger Name: tripartite motif-containing 31
• Berechnetes Molekulargewicht: 425 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC017017
• Gene symbol: TRIM31
• Gene ID (NCBI): 11074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.