TRIM33 Polyklonaler Antikörper

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TRIM33 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55374-1-PBS

Synonyme

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  WB analysis of COLO 320 using 55374-1-AP (same clone as 55374-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 55374-1-AP (same clone as 55374-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55374-1-AP (same clone as 55374-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55374-1-PBS bindet in WB, IHC, Indirect ELISA TRIM33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tripartite motif-containing 33
• Berechnetes Molekulargewicht: 122 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_015906
• Gene symbol: TRIM33
• Gene ID (NCBI): 51592
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).