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TRIM33 Rekombinanter Antikörper

TRIM33 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242728H2

Kat-Nr. : 85364-3-PBS

Synonyme

242728H2, E3 ubiquitin-protein ligase TRIM33, EC:2.3.2.27, Ectodermin homolog, KIAA1113

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM33 Recombinant monoclonal antibody (85364-3-RR)

    WB analysis using 85364-3-RR (same clone as 85364-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TRIM33 Recombinant monoclonal antibody (85364-3-RR)

    WB analysis of COLO 320 using 85364-3-RR (same clone as 85364-3-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TRIM33 Recombinant monoclonal antibody (85364-3-RR)

    IF Staining of A431 using 85364-3-RR (same clone as 85364-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85364-3-PBS bindet in WB, IF/ICC, Indirect ELISA TRIM33 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name tripartite motif-containing 33
Berechnetes Molekulargewicht 122 kDa
Beobachtetes Molekulargewicht140-150 kDa
GenBank-ZugangsnummerNM_015906
Gene symbol TRIM33
Gene ID (NCBI) 51592
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).