TRIM34 Rekombinanter Antikörper

TRIM34 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240180E2

Kat-Nr. : 83316-2-RR

Synonyme

IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34

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  WB analysis of K-562 using 83316-2-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 83316-2-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 83316-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83316-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83316-2-RR bindet in WB, IF/ICC, ELISA TRIM34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM34 fusion protein Ag34444
• Vollständiger Name: tripartite motif-containing 34
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 31 kDa, 57 kDa
• GenBank-Zugangsnummer: NM_001003827
• Gene symbol: TRIM34
• Gene ID (NCBI): 53840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).