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TRIM35 Polyklonaler Antikörper

TRIM35 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19806-1-PBS

Synonyme

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

Filter:
  • Western Blot (WB) analysis of A431 cells using TRIM35 Polyclonal antibody (19806-1-AP)

    WB analysis of A431 using 19806-1-AP (same clone as 19806-1-PBS)

    WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells. This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRIM35 Polyclonal antibody (19806-1-AP)

    WB analysis using 19806-1-AP (same clone as 19806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM35 Polyclonal antibody (19806-1-AP)

    IF Staining of U2OS using 19806-1-AP (same clone as 19806-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19806-1-PBS bindet in WB, IF/ICC, Indirect ELISA TRIM35 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRIM35 fusion protein Ag13584
Vollständiger Name tripartite motif-containing 35
Berechnetes Molekulargewicht 57 kDa
Beobachtetes Molekulargewicht45~50 kDa
GenBank-ZugangsnummerBC018337
Gene symbol TRIM35
Gene ID (NCBI) 23087
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.