TRIM47 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM47 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 26885-1-AP

Synonyme

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

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  WB analysis using 26885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26885-1-AP (TRIM47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26885-1-AP

  IP result of anti-TRIM47 (IP:26885-1-AP, 4ug; Detection:26885-1-AP 1:300) with U2OS cells lysate 2000ug.

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  IHC staining of human colon cancer using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe, U2OS-Zellen
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Nierengewebe, Maus-Kolongewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

26885-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA TRIM47 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM47 fusion protein Ag22868
• Vollständiger Name: tripartite motif-containing 47
• Berechnetes Molekulargewicht: 638 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC017299
• Gene symbol: TRIM47
• Gene ID (NCBI): 91107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Publikationen

Species	Application	Title
human	WB,IHC	Neoplasma; Trim47 overexpression correlates with poor prognosis in gastric cancer.; Authors - YuJian Xia
mouse,human	WB,IP,IHC	Signal Transduct Target Ther; TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.; Authors - Yisong Qian ; KD Validated
human	WB,IHC	In Vitro Cell Dev Biol Anim; TRIM47 promotes glioma angiogenesis by suppressing Smad4; Authors - Zifan Wang ; KD Validated
mouse,human	WB,IHC,IF	Clinics (Sao Paulo); TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling; Authors - Xi Wang ; KD Validated
rat	WB	Exp Brain Res; Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats; Authors - Yingjun Zhu ; KD Validated
human	WB,IF	Comput Struct Biotechnol J; Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress; Authors - Rui Su