TRIM47 Rekombinanter Antikörper

TRIM47 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251436A2

Kat-Nr. : 86728-1-PBS

Synonyme

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

---

• 

  WB analysis using 86728-1-RR (same clone as 86728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of OVCAR-3 using 86728-1-RR (same clone as 86728-1-PBS)

  OVCAR-3 cells were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86728-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86728-1-RR (same clone as 86728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86728-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

86728-1-PBS bindet in WB, Indirect ELISA TRIM47 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM47 fusion protein Ag22868
• Vollständiger Name: tripartite motif-containing 47
• Berechnetes Molekulargewicht: 638 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC017299
• Gene symbol: TRIM47
• Gene ID (NCBI): 91107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381).