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TRIM5 Monoklonaler Antikörper

TRIM5 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H1G9

Kat-Nr. : 67492-1-PBS

Synonyme

1H1G9, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM5, RNF88, tripartite motif containing 5

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM5 Monoclonal antibody (67492-1-Ig)

    WB analysis using 67492-1-Ig (same clone as 67492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67492-1-Ig (TRIM5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67492-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67492-1-PBS bindet in WB, Indirect ELISA TRIM5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen TRIM5 fusion protein Ag30059
Vollständiger Name tripartite motif-containing 5
Berechnetes Molekulargewicht 493 aa, 56 kDa
Beobachtetes Molekulargewicht 56 kDa
GenBank-ZugangsnummerBC021258
Gene symbol TRIM5
Gene ID (NCBI) 85363
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).