TRIM54 Rekombinanter Antikörper

TRIM54 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230391G5

Kat-Nr. : 83175-2-RR

Synonyme

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

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  WB analysis using 83175-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 83175-2-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83175-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 83175-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83175-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83175-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA TRIM54 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM54 fusion protein Ag15229
• Vollständiger Name: tripartite motif-containing 54
• Berechnetes Molekulargewicht: 358 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC141807
• Gene symbol: TRIM54
• Gene ID (NCBI): 57159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).