TRIM59 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM59 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28575-1-AP

Synonyme

MRF1, RING finger protein 104, RNF104, TRIM57, TRIM59, tripartite motif containing 59, TSBF1, Tumor suppressor TSBF 1

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  WB analysis using 28575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 28575-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 28575-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28575-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 28575-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28575-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens).

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  IF Staining of U2OS using 28575-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM59 antibody (28575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Mammakarzinomgewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

28575-1-AP bindet in WB, IHC, IF/ICC, CoIP, ChIP, ELISA TRIM59 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM59 fusion protein Ag29560
• Vollständiger Name: tripartite motif-containing 59
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: NM_173084
• Gene symbol: TRIM59
• Gene ID (NCBI): 286827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.

Publikationen

Species	Application	Title
human	CoIP,ChIP	J Transl Med; A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification; Authors - Yingli Liu
human	WB,CoIP	Heliyon; TRIM59-mediated ferroptosis enhances neuroblastoma development and chemosensitivity through p53 ubiquitination and degradation; Authors - Yingbei Liu ; KD Validated