TRIM62 Polyklonaler Antikörper

TRIM62 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15256-1-PBS

Synonyme

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  WB analysis using 15256-1-AP (same clone as 15256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15256-1-AP (same clone as 15256-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15256-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TRIM62 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM62 fusion protein Ag7143
• Vollständiger Name: tripartite motif-containing 62
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC001222
• Gene symbol: TRIM62
• Gene ID (NCBI): 55223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).