TRIM63 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM63 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(98)

Rezensionen (1)

Kat-Nr. : 55456-1-AP

Synonyme

MuRF1, MuRF 1, Iris RING finger protein, IRF, EC:2.3.2.27

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  WB analysis using 55456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55456-1-AP (TRIM63 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55456-1-AP (TRIM63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55456-1-AP (TRIM63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 55456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TRIM63 antibody (55456-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55456-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM63 antibody (55456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Mausherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 93 publications below
• IHC: See 8 publications below

Produktinformation

55456-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA TRIM63 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tripartite motif-containing 63
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_032588
• Gene symbol: TRIM63
• Gene ID (NCBI): 84676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
mouse	WB	J Cachexia Sarcopenia Muscle; Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.; Authors - Guo-Kai Shang
mouse	WB	J Cachexia Sarcopenia Muscle; Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors.; Authors - Guo-Kai Shang
mouse	WB	J Cachexia Sarcopenia Muscle; Serum insulin-like growth factor binding protein 2 levels as biomarker for pancreatic ductal adenocarcinoma-associated malnutrition and muscle wasting.; Authors - Jie Dong
mouse	WB	J Cachexia Sarcopenia Muscle; Nuciferine Attenuates Cancer Cachexia-Induced Muscle Wasting in Mice via HSP90AA1; Authors - Xueyan An
	WB	Int J Biol Macromol; Lycium barbarum polysaccharide mitigates high-fat-diet-induced skeletal muscle atrophy by promoting AMPK/PINK1/Parkin-mediated mitophagy; Authors - Yanru Ren

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brice-Emmanuel (Verified Customer) (10-12-2023)

I don't have the band at 40 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

TRIM63 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:55456-1-AP)