TRIM65 Polyklonaler Antikörper

TRIM65 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31990-1-AP

Synonyme

E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, Tripartite motif-containing protein 65

---

• 

  WB analysis using 31990-1-AP

  K-562 cells lysate was subjected to SDS PAGE followed by western blot with 31990-1-AP (TRIM65 antibody) at dilution of 1:1000 incubated at room temperature for 1 hour.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31990-1-AP bindet in WB, ELISA TRIM65 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM65 fusion protein Ag34639
• Vollständiger Name: tripartite motif-containing 65
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 70 kDa
• GenBank-Zugangsnummer: NM_173547
• Gene symbol: TRIM65
• Gene ID (NCBI): 201292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing protein 65 (TRIM65) is a member of the TRIM family of RING-type E3 ubiquitin ligases. It contains a RING-finger domain, a B-box domain, a coiled-coil domain, and a SPRY domain (PMID: 40473847). As an E3 ubiquitin ligase, TRIM65 is involved in the ubiquitination of substrate proteins, playing a role in various intracellular physiological processes, including intracellular signaling, protein degradation, cell proliferation, apoptosis, carcinogenesis, autophagy, and phenotypic transformation (PMID: 38939997). TRIM65 has been shown to promote ubiquitination and degradation of several proteins, such as TOX4, which is involved in apoptosis, and ARHGAP35, which is involved in cell migration (PMID: 38212319; 31332286). The 70 kDa band might be the result of SUMO modification.