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TRIM65 Polyklonaler Antikörper

TRIM65 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31990-1-PBS

Synonyme

E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, Tripartite motif-containing protein 65

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM65 Polyclonal antibody (31990-1-AP)

    WB analysis using 31990-1-AP (same clone as 31990-1-PBS)

    K-562 cells lysate was subjected to SDS PAGE followed by western blot with 31990-1-AP (TRIM65 antibody) at dilution of 1:1000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 31990-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31990-1-PBS bindet in WB, Indirect ELISA TRIM65 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRIM65 fusion protein Ag34639
Vollständiger Name tripartite motif-containing 65
Berechnetes Molekulargewicht57 kDa
Beobachtetes Molekulargewicht57 kDa, 70 kDa
GenBank-ZugangsnummerNM_173547
Gene symbol TRIM65
Gene ID (NCBI) 201292
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing protein 65 (TRIM65) is a member of the TRIM family of RING-type E3 ubiquitin ligases. It contains a RING-finger domain, a B-box domain, a coiled-coil domain, and a SPRY domain (PMID: 40473847). As an E3 ubiquitin ligase, TRIM65 is involved in the ubiquitination of substrate proteins, playing a role in various intracellular physiological processes, including intracellular signaling, protein degradation, cell proliferation, apoptosis, carcinogenesis, autophagy, and phenotypic transformation (PMID: 38939997). TRIM65 has been shown to promote ubiquitination and degradation of several proteins, such as TOX4, which is involved in apoptosis, and ARHGAP35, which is involved in cell migration (PMID: 38212319; 31332286). The 70 kDa band might be the result of SUMO modification.