TRIM72 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM72 Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 22151-1-AP

Synonyme

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

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  WB analysis using 22151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22151-1-AP (TRIM72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22151-1-AP

  IP result of anti-TRIM72 (IP:22151-1-AP, 4ug; Detection:22151-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

22151-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ELISA TRIM72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM72 fusion protein Ag17756
• Vollständiger Name: tripartite motif-containing 72
• Berechnetes Molekulargewicht: 477 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC033211
• Gene symbol: TRIM72
• Gene ID (NCBI): 493829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Int J Mol Sci; PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice; Authors - Baile Wu
mouse	WB	Int Immunopharmacol; MG53 binding to CAV3 facilitates activation of eNOS/NO signaling pathway to enhance the therapeutic benefits of bone marrow-derived mesenchymal stem cells in diabetic wound healing; Authors - Junwei Wu
human	WB,IP	Cancer Biomark; Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment; Authors - Xun Li ; KD Validated
rat	WB	Biochem Biophys Res Commun; Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway.; Authors - Jiang Li
rat	WB	BMC Cardiovasc Disord; Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner.; Authors - Jiawei Zhuang
human	WB	FASEB J; Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts; Authors - Yan Li ; KD Validated