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  • KD/KO Validated

TRIM8 Polyklonaler Antikörper

TRIM8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27463-1-AP

Synonyme

RNF27, RING-type E3 ubiquitin transferase TRIM8, Glioblastoma-expressed RING finger protein, GERP, EC:2.3.2.27



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, L02-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27463-1-AP bindet in WB, IHC, IF, IP, ELISA TRIM8 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TRIM8 fusion protein Ag26517
Vollständiger Name tripartite motif-containing 8
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht 61 kDa
GenBank-ZugangsnummerBC021925
Gene symbol TRIM8
Gene ID (NCBI) 81603
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TRIM8 antibody 27463-1-APProtokoll herunterladen
IHC protocol for TRIM8 antibody 27463-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF,IP

Oncogene

Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination

Authors - Lin Wang
  • KD Validated
humanWB

Sci Rep

Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.

Authors - Dachang Liu
humanWB,IF

Cancer Med

TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation

Authors - Fei Wu
  • KD Validated
humanWB,IHC

J Orthop Surg Res

The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma

Authors - Jian Wen
humanWB

Exp Mol Pathol

Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.

Authors - Lei Li
humanWB

Adipocyte

Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway

Authors - Mingxue Zhu
  • KD Validated