TRIM8 Polyklonaler Antikörper

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• KD/KO Validated

TRIM8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27463-1-AP

Synonyme

RNF27, RING-type E3 ubiquitin transferase TRIM8, Glioblastoma-expressed RING finger protein, GERP, EC:2.3.2.27

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  WB analysis of L02 using 27463-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27463-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

27463-1-AP bindet in WB, IHC, IF, IP, ELISA TRIM8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM8 fusion protein Ag26517
• Vollständiger Name: tripartite motif-containing 8
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC021925
• Gene symbol: TRIM8
• Gene ID (NCBI): 81603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).

Publikationen

Species	Application	Title
human	WB,IF,IP	Oncogene; Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination; Authors - Lin Wang ; KD Validated
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IF	Cancer Med; TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation; Authors - Fei Wu ; KD Validated
human	WB,IHC	J Orthop Surg Res; The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma; Authors - Jian Wen
human	WB	Exp Mol Pathol; Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.; Authors - Lei Li
human	WB	Adipocyte; Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway; Authors - Mingxue Zhu ; KD Validated