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TRIM8 Polyklonaler Antikörper
TRIM8 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 27463-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HepG2-Zellen, L02-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Produktinformation
27463-1-AP bindet in WB, IHC, IF, IP, ELISA TRIM8 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | TRIM8 fusion protein Ag26517 |
Vollständiger Name | tripartite motif-containing 8 |
Berechnetes Molekulargewicht | 61 kDa |
Beobachtetes Molekulargewicht | 61 kDa |
GenBank-Zugangsnummer | BC021925 |
Gene symbol | TRIM8 |
Gene ID (NCBI) | 81603 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TRIM8 antibody 27463-1-AP | Protokoll herunterladen |
IHC protocol for TRIM8 antibody 27463-1-AP | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Oncogene Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination
| ||
Sci Rep Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma. | ||
Cancer Med TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation
| ||
J Orthop Surg Res The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma | ||
Exp Mol Pathol Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis. | ||
Adipocyte Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway
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