TRIM9 Monoklonaler Antikörper

TRIM9 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2B10

Kat-Nr. : 67268-1-PBS

Synonyme

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

---

• 

  WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67268-1-PBS bindet in WB, IHC, Indirect ELISA TRIM9 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM9 fusion protein Ag27856
• Vollständiger Name: tripartite motif-containing 9
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 79 kDa, 61 kDa
• GenBank-Zugangsnummer: BC013414
• Gene symbol: TRIM9
• Gene ID (NCBI): 114088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.