TRIP13 Monoklonaler Antikörper

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• KD/KO Validated

TRIP13 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1F1E9

Kat-Nr. : 67759-1-Ig

Synonyme

16E1 BP, 16E1BP, TR interacting protein 13, TRIP 13, TRIP13

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  WB analysis using 67759-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67759-1-Ig (TRIP13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67759-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67759-1-Ig (TRIP13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

67759-1-Ig bindet in WB, IHC, ELISA TRIP13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIP13 fusion protein Ag6595
• Vollständiger Name: thyroid hormone receptor interactor 13
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC000404
• Gene symbol: TRIP13
• Gene ID (NCBI): 9319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIP13 gene encodes thyroid receptor-interacting protein 13, that interact with thyroid hormone receptors, also known as human papillomavirus type 16 E1 protein-binding protein (16E1BP). This protein belongs to the AAA ATPase family and the PCH2 subfamily. TRIP13 gene may be one of the several playing roles in early-stage non-small cell lung cancer. TRIP13 also functions in chromosome recombination and chromosome structure development during meiosis, and it is required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation.

Publikationen

Species	Application	Title
human	WB	J Adv Res; Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer; Authors - Yu-Jie Sun
human	WB	Environ Toxicol; TRIP13 exerts a cancer-promoting role in cervical cancer by enhancing Wnt/β-catenin signaling via ACTN4; Authors - Xiaoying Liu ; KD Validated
human,mouse	WB,IHC	ACS Omega; TRIP13 Plays an Important Role in the Sensitivity of Leukemia Cell Response to Sulforaphane Therapy; Authors - Lei Liu