TRIP4 Polyklonaler Antikörper

TRIP4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12324-1-PBS

Synonyme

ASC 1, ASC-1, HsT17391, RQT4, Thyroid receptor-interacting protein 4

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  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 12324-1-AP (same clone as 12324-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12324-1-PBS bindet in WB, IHC, Indirect ELISA TRIP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIP4 fusion protein Ag2987
• Vollständiger Name: thyroid hormone receptor interactor 4
• Berechnetes Molekulargewicht: 581 aa, 66 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC012448
• Gene symbol: TRIP4
• Gene ID (NCBI): 9325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)