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TRMT1 Rekombinanter Antikörper

TRMT1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250178D5

Kat-Nr. : 85897-5-PBS

Synonyme

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

Filter:
  • Western Blot (WB) analysis of various lysates using TRMT1 Recombinant antibody (85897-5-RR)

    WB analysis using 85897-5-RR (same clone as 85897-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRMT1 Recombinant antibody (85897-5-RR)

    IF Staining of HeLa using 85897-5-RR (same clone as 85897-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85897-5-RR

    Affinity and Kinetic Characterization of 85897-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

Geprüfte Anwendungen

Produktinformation

85897-5-PBS bindet in WB, IF/ICC, Indirect ELISA TRMT1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TRMT1 fusion protein Ag6837
Vollständiger Name TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 81 kDa
Beobachtetes Molekulargewicht75 kDa
GenBank-ZugangsnummerBC002492
Gene symbol TRMT1
Gene ID (NCBI) 55621
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.